 |
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
|
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
| Cell line name | HUVEC |
| Accession number | ICLC IST05001 |
| Brief description | Species: human; Tissue: endothelium |
| Description | Species: human; Tissue: endothelium; Derived from: umbilical cord vein |
| Depositor | Dr A. Albini, Exp. Oncology A Dept, IST, Genoa, Italy |
| Reference paper | Microvasc Res 2004;68(2):110-8 - DOI: 10.1016/j.mvr.2004.05.001 - PMID: 15313120 |
| Morphology and growth | primary culture, grown as monolayer, morphology endothelial |
| Culture conditions | Medium 199 + 10% FBS + 2mM L-Glutamine + heparin 100 mg/L + aFGF 10ug/L + bFGF 10ug/L + EGF 10ug/L + hydrocortisone 1mg/L; split the cells 1:2-1:3 using cold trypsin/EDTA (no more than one minute), seed at 6x10^3 cells/cm2, 37° C, 5% CO2 |
| Properties | % of CD31 positive cells: >90% |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Comments | The cells reach at least passage 12; better results are guaranteed at low passages. Each ampoule contains about 5x10^5 cells. After thawing the viability of the cells may be low. The medium should be kept at 4°C, it should never be heated neither left too long at room temperature. The flasks must be presoaked with gelatin 1% in PBS for 30 minutes. After gelatin coating, rinse once with cold PBS. Do not reuse flask. Donors are tested for HBV, HCV, HIV. HUVEC are available from single donors (male or female), and the sex is specified. |