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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | Y79 |
| Accession number | ICLC HTL99029 |
| Brief description | Species: human, Caucasian; Tumor: retinoblastoma |
| Description | Species: human, Caucasian female 2 years old; Tumor: retinoblastoma |
| Depositor | Dr F. Tosetti, Molecular Biology Dept, IST at ABC, Genoa, Italy |
| Reference paper | J Natl Cancer Inst 1974;53:347-360 - DOI: 10.1093/jnci/53.2.347 - PMID: 4135597 |
| Morphology and growth | continuous culture, grown in suspension, morphology multicellular clusters |
| Culture conditions | RPMI 1640 + 2mM L-Glutamine + 15% FBS; maintain cultures between 5-9 x 10^5 cells/ml; split 1:2-1:4 (not disaggregate the clusters); 37C, 5% CO2. After thawing the viability of the cells may be low (thaw with medium supplemented with 20% FBS). |
| Properties | IRBP |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Comments | In order to obtain a cell monolayer the surface of the flask must be coated with poly-D-lysine 2.5 ug/cm2 |