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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | HT-29 |
| Accession number | ICLC HTL99026 |
| Brief description | Species: human, Caucasian; Tissue: colon; Tumor: adenocarcinoma, grade II |
| Description | Species: human, Caucasian female 44 years old; Tissue: colon; Tumor: adenocarcinoma, grade II |
| Depositor | Dr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy |
| Reference paper | Human tumor cells in vitro. Fogh J. ed., Plenum Press, New York, 1975; pp. 115-159 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | McCoy's 5A + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | CEA production. HLA: A1, A3; B12, B17; Cw5. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Tumorigenicity | tumorigenic |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Amelogenin: X; vWA: 17,19; FGA: 20,22; TH01: 6,9; D18S51: 13,9.2; D21S11: 29,30; D8S1179: 10,16 |