Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | KARPAS-422 |
Accession number | ICLC HTL99023 |
Brief description | Species: human; Tumor: lymphoma, follicular, B cell |
Description | Species: human female 73 years old; Tumor: lymphoma, follicular, B cell |
Depositor | Dr G. Canti, Pharmacology Dept, Milan, Italy |
Reference paper | Blood 1990;75:709-714 - PMID: 2297573 |
Morphology and growth | continuous culture, grown in suspension, morphology round to polygonal cells, growing in single or in small clusters |
Culture conditions | RPMI 1640 + 20% FBS + 2mM L-Glutamine; maintain cultures between 5-10 x 10^5 cells/ml; split 1:3-1:6; 37C, 5% CO2 |
Viruses | Epstein-Barr |
Properties | IgH/bcl2 fusion gene; CD3- CD10+ CD13(+) CD19+ CD20+ CD34+ CD37+ EBV+ |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
Karyology | (14;18)(q32,q21) chromosomal translocation |
Freezing medium | 90% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
Comments | the cells are difficult to culture; viability may be low after thawing |