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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SW48 |
| Accession number | ICLC HTL99020 |
| Brief description | Species: human, Caucasian; Tissue: colon; Tumor: adenocarcinoma, grade IV |
| Description | Species: human, Caucasian female 82 years old; Tissue: colon; Tumor: adenocarcinoma, grade IV |
| Depositor | Dr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy |
| Reference paper | Cancer Res 1976;36:4562-4569 - PMID: 1000501 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | L-15 + 10% FBS + 2mM L-Glutamine; split confluent culture 1:3-1:4 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; incubate at 37°C without CO2 (close the flask caps) |
| Properties | HLA: A23, A33; B27, B35 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | J Natl Cancer Inst 1977;59:221-226 - DOI: 10.1093/jnci/59.1.221
- PMID: 327080
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| Comments | the cells are difficult to detach: at least 15 min. in trypsin or scraper are required |