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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | BV-173 |
| Accession number | ICLC HTL99018 |
| Brief description | Species: human; Tissue: peripheral blood; Tumor: leukemia, pre-B cell |
| Description | Species: human male 45 years old; Tissue: peripheral blood; Tumor: leukemia, pre-B cell |
| Depositor | Dr L. Pegoraro, Cytogenetics and Molecular Biology Dept, Turin, Italy |
| Reference paper | J Natl Cancer Inst 1983;70:447-453 - PMID: 6572735 |
| Morphology and growth | continuous culture, grown in suspension, morphology small round cells |
| Culture conditions | RPMI 1640 + 20% FBS + 2mM L-Glutamine; maintain cultures between 0.5 - 1 x 10^6 cells/ml; 37C, 5% CO2 |
| Viruses | Epstein-Barr |
| Properties | The cells express bcl2 and c-myc mRNA |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Comments | the cells grow slowly, are quite difficult to culture and should be frozen at not less than 5 x 10^6 cells/ampoule |