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Interlab Cell Line Collection (ICLC)
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Cell line nameSW480
Accession numberICLC HTL99017
Brief descriptionSpecies: human; Tissue: colon; Tumor: adenocarcinoma, grade III-IV
DescriptionSpecies: human; Tissue: colon; Tumor: adenocarcinoma, grade III-IV
DepositorDr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy
Reference paperCancer Res 1976;36:4562-4569 - PMID: 1000501
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like, some round cells
Culture conditionsL-15 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-3 x 10^4 cells/cm2; incubate at 37°C without CO2 (close the flask caps)
PropertiesCEA production. HLA: A2; B8, B17
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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