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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | DU-145 |
| Accession number | ICLC HTL99013 |
| Brief description | Species: human, Caucasian; Tissue: prostate; Tumor: carcinoma |
| Description | Species: human, Caucasian male 69 years old; Tissue: prostate; Tumor: carcinoma; Derived from: metastasis to brain |
| Depositor | Dr P. Limonta, Institute of Endocrinology, Milan, Italy |
| Reference paper | Int J Cancer 1978, 21:274-281 - DOI: 10.1002/ijc.2910210305 - PMID: 631930 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | RPMI 1640 + 5-10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Passage number | 67 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Amelogenin: X,Y; vWA: 17,18; FGA: 22; TH01: 7; D18S51: 12; D21S11: 29,30,33,71; D8S1179: 13,14 |
| Further bibliography | Cancer Res 1977, 37:4049-4058 - PMID: 908039
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