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Cell line nameDU-145
Accession numberICLC HTL99013
Brief descriptionSpecies: human, Caucasian; Tissue: prostate; Tumor: carcinoma
DescriptionSpecies: human, Caucasian male 69 years old; Tissue: prostate; Tumor: carcinoma; Derived from: metastasis to brain
DepositorDr P. Limonta, Institute of Endocrinology, Milan, Italy
Reference paperInt J Cancer 1978, 21:274-281 - DOI: 10.1002/ijc.2910210305 - PMID: 631930
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsRPMI 1640 + 5-10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Passage number67
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Amelogenin: X,Y; vWA: 17,18; FGA: 22; TH01: 7; D18S51: 12; D21S11: 29,30,33,71; D8S1179: 13,14
Further bibliographyCancer Res 1977, 37:4049-4058 - PMID: 908039

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