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Interlab Cell Line Collection (ICLC)
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Cell line nameSK-MEL-28
Accession numberICLC HTL99010
Brief descriptionSpecies: human; Tissue: skin; Tumor: melanoma
DescriptionSpecies: human male 51 years old; Tissue: skin; Tumor: melanoma
DepositorProf. S. Toma, Oncology, Biology and Genetic Dept, University of Genoa, Italy
Reference paperProc Natl Acad Sci USA 1976;73:3278-3282 - DOI: 10.1073/pnas.73.9.3278 - PMID: 1067619
Morphology and growthcontinuous culture, grown as monolayer, morphology polygonal cells
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2
PropertiesHLA: A11, A26; B40; DRw4
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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