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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SK-MEL-28 |
| Accession number | ICLC HTL99010 |
| Brief description | Species: human; Tissue: skin; Tumor: melanoma |
| Description | Species: human male 51 years old; Tissue: skin; Tumor: melanoma |
| Depositor | Prof. S. Toma, Oncology, Biology and Genetic Dept, University of Genoa, Italy |
| Reference paper | Proc Natl Acad Sci USA 1976;73:3278-3282 - DOI: 10.1073/pnas.73.9.3278 - PMID: 1067619 |
| Morphology and growth | continuous culture, grown as monolayer, morphology polygonal cells |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2 |
| Properties | HLA: A11, A26; B40; DRw4 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |