Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | SW1353 |
Accession number | ICLC HTL99008 |
Brief description | Species: human, Caucasian; Tissue: bone; Tumor: chondrosarcoma |
Description | Species: human, Caucasian female 72 years old; Tissue: bone; Tumor: chondrosarcoma |
Depositor | Dr T. Florio, Neuroscience Unit, ABC, Genoa, Italy |
Reference paper | Unknown |
Morphology and growth | continuous culture, grown as monolayer, morphology large cells, fibroblast-like |
Culture conditions | L-15 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:2-1:3 weekly using trypsin/EDTA; incubate at 37°C without CO2 (close the flask caps) |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |