Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | ZR-75-1 |
Accession number | ICLC HTL99006 |
Brief description | Species: human, Caucasian; Tissue: breast; Tumor: carcinoma, ductal |
Description | Species: human, Caucasian female 63 years old; Tissue: breast; Tumor: carcinoma, ductal |
Depositor | Prof. S. Toma, Oncology, Biology and Genetic Dept, University of Genoa, Italy |
Reference paper | Cancer Res 1978;38:3352-3364 - PMID: 688225 |
Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
Properties | positive for estrogen receptor (ER+) |
Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
Further bibliography | Cancer Res 1978;38:4327-4339 - PMID: 212193
Int J Food Sci Nutr 2000;51:S95-103 - PMID: 11271861 |