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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | HCT-8 |
| Accession number | ICLC HTL99005 |
| Brief description | Species: human; Tissue: intestine, ileocecal; Tumor: adenocarcinoma |
| Description | Species: human male 67 years old; Tissue: intestine, ileocecal; Tumor: adenocarcinoma |
| Depositor | Dr A. Sobrero, Udine, Italy |
| Reference paper | J Natl Cancer Inst 1974;52:1101-1110 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | RPMI 1640 + 10% HS + 2mM L-Glutamine; split confluent cultures 1:5-1:20 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | CEA production; the cell line was demonstrated by cytogenetic analysis to be identical to HRT-18 cell line |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Int J Cancer 1975;16:74-82 |