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Interlab Cell Line Collection (ICLC)
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Cell line nameMDA-MB-231
Accession numberICLC HTL99004
Brief descriptionSpecies: human, Caucasian; Tissue: breast; Tumor: adenocarcinoma
DescriptionSpecies: human, Caucasian female 51 years old; Tissue: breast; Tumor: adenocarcinoma; Derived from: pleural effusion
DepositorProf. S. Toma, Oncology, Biology and Genetic Dept, University of Genoa, Italy
Reference paperJ Natl Cancer Inst 1974;53:661-674 - DOI: 10.1093/jnci/53.3.661 - PMID: 4412247
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
Propertiesnegative for estrogen receptors (ER-)
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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