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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | MG-63 |
| Accession number | ICLC HTL99003 |
| Brief description | Species: human; Tumor: osteosarcoma |
| Description | Species: human male 14 years old; Tumor: osteosarcoma |
| Depositor | Prof. A. Colombatti, Centro di Riferimento Oncologico, Aviano, Italy |
| Reference paper | Antimicrob Agents Chemother 1977;12:11-15 - DOI: 10.1128/AAC.12.1.11 - PMID: 883813 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | the cells are reported to produce high levels of interferon following superinduction with polyinosinic acid-polycytidilic acid, cycloheximide and actinomycin D |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |