Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | A-204 |
Accession number | ICLC HTL99002 |
Brief description | Species: human; Tissue: muscle; Tumor: rhabdomyosarcoma |
Description | Species: human female 1 year old; Tissue: muscle; Tumor: rhabdomyosarcoma |
Depositor | Prof. A. Colombatti, Centro di Riferimento Oncologico, Aviano, Italy |
Reference paper | J Natl Cancer Inst 1973;51:1417-1423 - DOI: 10.1093/jnci/51.5.1417 - PMID: 4357758 |
Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2 -4x10^4 cells/cm2; 37C, 5% CO2 |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
Comments | The cells are difficult to detach |