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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | G-361 |
| Accession number | ICLC HTL99001 |
| Brief description | Species: human; Tissue: skin; Tumor: melanoma |
| Description | Species: human male 31 years old; Tissue: skin; Tumor: melanoma |
| Depositor | Dr S. Barlati, Biology and Genetics Dept, University of Brescia, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4x10^4 cells/cm2;37C, 5% CO2 |
| Properties | The cells produce tissue-type plasminogen activator (tPA) |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | FEBS Lett 1995;363:170-174 - DOI: 10.1016/0014-5793(95)00312-w
- PMID: 7537224
Invasion Metastasis 1995;15:22-23 - PMID: 7672929 Pediatr Res 1978;12:485 |