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Interlab Cell Line Collection (ICLC)
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Cell line nameCaSki
Accession numberICLC HTL98018
Brief descriptionSpecies: human, Caucasian; Tissue: cervix; Tumor: carcinoma, epidermoid, from metastatic site
DescriptionSpecies: human, Caucasian female 40 years old; Tissue: cervix; Tumor: carcinoma, epidermoid, from metastatic site
DepositorDr I. Bongarzone, Istituto Nazionale Tumori, Milan, Italy
Reference paperScience 1977;196:1456-1458
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x10^4 cells/cm2; 37C, 5% CO2
PropertiesThe cells have reported to secrete the beta subunit of human chorionic gonadotropin (beta-hCG) and express tumor-associated antigen
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle under Biosafety Level 2 containment
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Passage number253
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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