| Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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| Cell line name | A2780 |
| Accession number | ICLC HTL98008 |
| Brief description | Species: human; Tissue: ovary; Tumor: adenocarcinoma |
| Description | Species: human; Tissue: ovary; Tumor: adenocarcinoma |
| Depositor | Obtained from ECACC |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3 - 1:6 using trypsin/EDTA; seed at 3-6x10^4 cells/cm2; 37C, 5% CO2 |
| Properties | toxicity testing, cancer genetic studies |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
| Validated by HLA-DP beta: confirmed as human by PCR | |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1985;45:2110-2115 - PMID: 3986765
Cancer Res 1987;47:414 - PMID: 3539322 Semin Oncol 1984;11:285 |