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Cell line nameDBTRG-05MG
Accession numberICLC HTL98001
Brief descriptionSpecies: human; Tissue: brain; Tumor: glioblastoma multiforme
DescriptionSpecies: human female; Tissue: brain; Tumor: glioblastoma multiforme
DepositorObtained from ECACC.
Reference paperIn Vitro 1992;28A:609 - DOI: 10.1007/BF02631035 - PMID: 1331021
Morphology and growthcontinuous culture, grown as monolayer, morphology glial
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2
PropertiesHLA class I expressed
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Karyology2n=87-91; hypotetraploid
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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