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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | TE671 Subline No.2 |
| Accession number | ICLC HTL97021 |
| Brief description | Species: human, Caucasian; Tumor: rhabdomyosarcoma |
| Description | Species: human, Caucasian female 6 years old; Tumor: rhabdomyosarcoma; Derived from: TE671 (described as medulloblastoma, then confirmed as rhabdomyosarcoma, probably identical to RD). |
| Depositor | Obtained from ECACC. |
| Reference paper | Int J Cancer 1977;20:206-212 - DOI: 10.1002/ijc.2910200207 - PMID: 892931 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; Seed at 2-4 x 104 cells/cm2; split confluent cultures 1:3-1:6 using trypsin/EDTA. 37C, 5% CO2. |
| Properties | nicotinic acetylcholine receptor; acetylcholine receptor; peripheral type benzodiazepine receptor. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, G6PD |
| Passage number | 10 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Brain Res 1982;231:365-367 - DOI: 10.1016/0006-8993(82)90373-0
- PMID: 7055685
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