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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SK-N-F1 |
| Accession number | ICLC HTL97020 |
| Brief description | Species: human; Tumor: neuroblastoma |
| Description | Species: human male; Tumor: neuroblastoma; Derived from: bone marrow metastasis |
| Depositor | Obtained from ECACC. |
| Reference paper | J Natl Cancer Inst 1984;73(1):51 - PMID: 6610792 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like, clumped cells tend to float |
| Culture conditions | DMEM + 10% FBS + 1% non-essential amino acids + 4mM L-Glutamine; Split confluent coltures 1:3-1:5 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2. |
| Properties | transferrin receptor; choline acetyltransferase synthesis; carcinogenesis studies; cytotoxicity assays; neuroblastoma specific peptide secretion. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
| Karyology | aneuploid |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Anticancer Drugs 1993;4:487 - DOI: 10.1097/00001813-199308000-00010
- PMID: 8104544
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