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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | MEWO |
| Accession number | ICLC HTL97019 |
| Brief description | Species: human; Tumor: malignant melanoma |
| Description | Species: human; Tumor: malignant melanoma |
| Depositor | Obtained from ECACC. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2 |
| Properties | melanin production; virology; Susceptible to: varicella zoster |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Infect Immun 1978;19:199-203 - DOI: 10.1128/iai.19.1.199-203.1978
- PMID: 203532
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| Comments | Susceptible to varicella zoster: optimal growth 32C |