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Interlab Cell Line Collection (ICLC)
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Cell line namePC-3
Accession numberICLC HTL97018
Brief descriptionSpecies: human, Caucasian; Tissue: prostate; Tumor: adenocarcinoma, grade IV
DescriptionSpecies: human, Caucasian male 62 years old; Tissue: prostate; Tumor: adenocarcinoma, grade IV
DepositorObtained from ECACC.
Reference paperInvest Urol 1979;17:16 - PMID: 447482
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsHam's F12 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-5x104 cells/cm2; 37C, 5% CO2. Initially after thawing the viability of the cells may be low, the cells grow slowly and loosely adherent.
PropertiesHLA: A1; A9
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Passage number29
Tumorigenicitytumorigenic in nude mice
Karyologytriploid, modal number 62
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1980;40:524 - PMID: 7471073

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