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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | PC-3 |
| Accession number | ICLC HTL97018 |
| Brief description | Species: human, Caucasian; Tissue: prostate; Tumor: adenocarcinoma, grade IV |
| Description | Species: human, Caucasian male 62 years old; Tissue: prostate; Tumor: adenocarcinoma, grade IV |
| Depositor | Obtained from ECACC. |
| Reference paper | Invest Urol 1979;17:16 - PMID: 447482 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | Ham's F12 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-5x104 cells/cm2; 37C, 5% CO2. Initially after thawing the viability of the cells may be low, the cells grow slowly and loosely adherent. |
| Properties | HLA: A1; A9 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
| Passage number | 29 |
| Tumorigenicity | tumorigenic in nude mice |
| Karyology | triploid, modal number 62 |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1980;40:524 - PMID: 7471073
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