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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | FTC-133 |
| Accession number | ICLC HTL97015 |
| Brief description | Species: human; Tissue: thyroid; Tumor: carcinoma, follicular |
| Description | Species: human male 42 years old; Tissue: thyroid; Tumor: carcinoma, follicular; Derived from: a lymph node metastasis |
| Depositor | Obtained from ECACC. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology polygonal flat to spindle-like |
| Culture conditions | DMEM + Ham's F12 (1:1) + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-5x104 cells/cm2. 37C, 5% CO2. |
| Properties | The cell line has been used for studies on the function of human thyroid and development of thyroid cancer; expression of 5-deiodinase type I; the cells retained differentiated thyrocyte function and thyrocyte responsiveness to thyrotropin and local active growth factors; thyroglobulin immunoreactivity could be shown in the cytoplasm and EGF receptor immunoreactivity could be visualised on the cell membranes |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with G6PD, NP. |
| Passage number | 12 |
| Karyology | complex chromosomal changes; p53 mutation |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Front Horm Res 1989;18:56 - DOI: 10.1016/0006-291x(75)90293-4 |