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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | THP-1 |
| Accession number | ICLC HTL97014 |
| Brief description | Species: human; Tissue: peripheral blood; Tumor: leukemia, acute monocytic |
| Description | Species: human male 1 years old; Tissue: peripheral blood; Tumor: leukemia, acute monocytic |
| Depositor | Dr A. Rubartelli, Clinical Pathology Dept, IST, Genoa, Italy. |
| Reference paper | Int J Cancer 1980;26:171-176 - DOI: 10.1002/ijc.2910260208 - PMID: 6970727 |
| Morphology and growth | continuous culture, grown in suspension, morphology large, round, single cells |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Maintain cultures between 2-9x105 cells/ml. 37C, 5% CO2 |
| Properties | differentiation; lysozyme synthesis; phagocytosis; Fc receptor; IL-1 production; C3b receptor. HLA: A2, A9; B5; DRw1, DRw2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with G6PD, NP |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Amelogenin: X,Y; vWA: 16; FGA: 24,25; TH01: 8,9.3; D18S51: 13,14; D21S11: 30,31.2; D8S1179: 10,14 |
| Further bibliography | Cancer Res 1982;42:1530 - PMID: 6949641
J Immunol 1983;131:1882 - PMID: 6619543 |