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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | A-172 |
| Accession number | ICLC HTL97013 |
| Brief description | Species: human; Tumor: glioblastoma |
| Description | Species: human male 53 years old; Tumor: glioblastoma |
| Depositor | Obtained from ECACC. |
| Reference paper | J Natl Cancer Inst 1973;51:1417-1423 - DOI: 10.1093/jnci/51.5.1417 - PMID: 4357758 |
| Morphology and growth | continuous culture, grown as monolayer, morphology glial |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; Seed at 2-4x104 cells/cm2; split confluent cultures 1:4-1:8 using trypsin/EDTA. 37C, 5% CO2. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with G6PD, NP |
| Passage number | 318 |
| Tumorigenicity | non tumorigenic in anti thymocyte |
| Karyology | hypertriploid; n = 80, over 20 marker chromosomes per cell |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |