 |
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
|
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
| Cell line name | IST-SL2 |
| Accession number | ICLC HTL97010 |
| Brief description | Species: human; Tissue: lung; Tumor: carcinoma, small cell |
| Description | Species: human male; Tissue: lung; Tumor: carcinoma, small cell; Derived from: pleural effusion |
| Depositor | Dr S. Ferrini, Immunopharmacology Dept, IST at ABC, Genoa, Italy. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown in suspension, morphology round cells, clusters |
| Culture conditions | RPMI 1640 + 10-20% FBS + 2mM L-Glutamine; maintain cultures between 2 - 4 x 10^5 cells/ml; split cultures 1:2-1:3; 37C, 5% CO2. |
| Properties | negative for HLA antigens |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP. |
| Passage number | 67 |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Comments | After thawing, the cells appear very suffering, and grow slowly (thaw with medium supplemented with FBS 20%) |