Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | CAS-1 |
Accession number | ICLC HTL97009 |
Brief description | Species: human; Tumor: glioblastoma |
Description | Species: human male 63 years old; Tumor: glioblastoma |
Depositor | Dr T. Florio, Neuroscience Unit, ABC, Genoa, Italy |
Reference paper | Unknown |
Morphology and growth | continuous culture, grown as monolayer, morphology glial |
Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |