Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | PSN1 |
Accession number | ICLC HTL97008 |
Brief description | Species: human; Tissue: pancreas; Tumor: adenocarcinoma |
Description | Species: human; Tissue: pancreas; Tumor: adenocarcinoma |
Depositor | Obtained from ECACC. |
Reference paper | Biochem Biophys Res Commun 1986;140:167 - DOI: 10.1016/0006-291x(86)91072-7 - PMID: 3778442 |
Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:8 using trypsin/EDTA; seed at 1-3 x 104 cells/cm2. 37C, 5% CO2. |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
Passage number | 9 |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
Further bibliography | Cancer Res 1991;51:3356 - PMID: 2054775
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