Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | CA46 |
Accession number | ICLC HTL97007 |
Brief description | Species: human; Tissue: ascitic fluid; Tumor: lymphoma, Burkitt |
Description | Species: human; Tissue: ascitic fluid; Tumor: lymphoma, Burkitt |
Depositor | Obtained from ECACC. |
Reference paper | J Natl Cancer Inst 1980;64:477-483 - PMID: 6243722 |
Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast-like |
Culture conditions | RPMI 1640 + 20% FBS + 2mM L-Glutamine; Maintain cultures between 5 and 9 x 105 cells/ml. 37C, 5% CO2 |
Properties | bcl-2 mRNA expression; c-myc mRNA expression; complement receptor; surface IgM (kappa) expression; IgM secretion; EBNA negative |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by isoenzymes: confimed as human with MD,NP. |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
Further bibliography | J Immunol 1982;129:1336-1342 - PMID: 6286763
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