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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | PA-1 |
| Accession number | ICLC HTL97002 |
| Brief description | Species: human, Caucasian; Tissue: ovary; Tumor: teratocarcinoma |
| Description | Species: human, Caucasian female 12 years old; Tissue: ovary; Tumor: teratocarcinoma; Derived from: ascitic fluid |
| Depositor | Dr P. Russo, Experimental Oncology Dept, IST, Genoa, Italy. |
| Reference paper | Int J Cancer 1980;25:19-32 - DOI: 10.1002/ijc.2910250104 - PMID: 6931103 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:10 using trypsin/EDTA; seed at 1-3x104 cells/cm2; 37C, 5% CO2. |
| Properties | HLA expression: A28; B12. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with AST, G6PD |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | In Vitro 1974;10:382 - DOI: 10.1016/0006-291x(75)90293-4
J Natl Cancer Inst 1974;52:921 - DOI: 10.1093/jnci/52.3.921
- PMID: 4524119
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