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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | 8305C |
| Accession number | ICLC HTL96026 |
| Brief description | Species: human; Tissue: thyroid; Tumor: carcinoma, undifferentiated |
| Description | Species: human female 67 years old; Tissue: thyroid; Tumor: carcinoma, undifferentiated |
| Depositor | Obtained from ECACC. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology large spindle-shaped cells |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:4 once a week using trypsin/EDTA. Doubling time is about 43 hours. Seed at 2-4 x 104 cells/cm2; cell harvest is about 2 x 106 cells/150 cm2 flask. 37C, 5% CO2. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with AST, MD |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Biochem Biophys Res Commun 1994;198:948-954 - DOI: 10.1006/bbrc.1994.1135
- PMID: 8117301
Cancer Res 1992;52:1369 - PMID: 1737400 Int J Oncol 1994;4:583-586 - DOI: 10.1016/0006-291x(75)90293-4 |