Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | SK-LU-1 |
Accession number | ICLC HTL96023 |
Brief description | Species: human, Caucasian; Tissue: lung; Tumor: adenocarcinoma, grade III, poorly differentiated |
Description | Species: human, Caucasian female 60 years old; Tissue: lung; Tumor: adenocarcinoma, grade III, poorly differentiated |
Depositor | Obtained from ECACC. |
Reference paper | Unknown |
Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 3-5x104 cells/cm2.37C, 5% CO2. |
Properties | HLA phenotype Aw24,Aw32,B27,Bw41 |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by isoenzymes: confirmed as human with AST, G6PD |
Tumorigenicity | tumorigenic in immunotolerant rats |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |