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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SK-N-SH |
| Accession number | ICLC HTL96019 |
| Brief description | Species: human, Caucasian; Tumor: neuroblastoma |
| Description | Species: human, Caucasian female 4 years old; Tumor: neuroblastoma; Derived from: bone marrow metastasis |
| Depositor | Dr P. Montaldo, G. Gaslini Institute, Genoa, Italy. |
| Reference paper | In Vitro 1973;7:410 - DOI: 10.1016/0006-291x(75)90293-4 |
| Morphology and growth | continuous culture, grown as monolayer, morphology neuroblast-like and epithelial-like |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2. |
| Properties | receptor cell line in cell-mediated cytotoxicity |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with AST, G6PD |
| Tumorigenicity | tumorigenic in nude mice |
| Karyology | modal number 47 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1973;33:2643-2652 - PMID: 4748425
Proc Natl Acad Sci USA 1978;75:3965-3969 - DOI: 10.1073/pnas.75.8.3965 - PMID: 279013 |