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Cell line nameMEG-01
Accession numberICLC HTL96017
Brief descriptionSpecies: human; Tissue: bone marrow; Tumor: leukemia, megakaryoblastic
DescriptionSpecies: human male 55 years old; Tissue: bone marrow; Tumor: leukemia, megakaryoblastic; Derived from: a patient with blast crisis of Philadelphia (Ph1) chromosome + chronic myelogenous leukemia (CML)
DepositorObtained from ECACC.
Reference paperBlood 1985;66:1384-1392 - PMID: 2998511
Morphology and growthcontinuous culture, grown in suspension, morphology mixed, round and some adherent cells
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:5-1:10 using trypsin/EDTA; maintain cultures between 2-4x105 cells/ml; 37C, 5% CO2.
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with AST, G6PD, PepB
Karyologymodal Nø 56-58; hyperdiploid
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyExp Cell Res 1991;193:223 - DOI: 10.1016/0014-4827(91)90560-h - PMID: 1995298

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