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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SK-N-BE(2) |
| Accession number | ICLC HTL96015 |
| Brief description | Species: human, Caucasian; Tissue: bone marrow; Tumor: neuroblastoma |
| Description | Species: human, Caucasian male 22 months old; Tissue: bone marrow; Tumor: neuroblastoma |
| Depositor | Obtained from ECACC |
| Reference paper | Science 1976;191:185-187 - DOI: 10.1126/science.942798 - PMID: 942798 |
| Morphology and growth | continuous culture, grown as monolayer, morphology neuroblast-like and epithelial-like, at confluence forms loosely adherent aggregates |
| Culture conditions | MEM (EBSS) + Ham's F12 (1:1) + 10% FBS + 2 mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2. |
| Properties | the cells are multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with G6PD, MD, NP |
| Passage number | 29 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1978;38:3751-3757 - PMID: 29704
J Natl Cancer Inst 1976;57:683-695 - DOI: 10.1093/jnci/57.3.683 - PMID: 62055 Life Sci 1991;50:7-11 - DOI: 10.1016/0006-291x(75)90293-4 |