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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | NCI-H292 |
| Accession number | ICLC HTL96009 |
| Brief description | Species: human, Black; Tissue: lung; Tumor: carcinoma, mucoepidermoid |
| Description | Species: human, Black female 32 years old; Tissue: lung; Tumor: carcinoma, mucoepidermoid; Derived from: lymph node metastasis |
| Depositor | Obtained from ECACC |
| Reference paper | Cancer Res 1985;45:2913-2923 - PMID: 2985257 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-6 x 104 cells/cm2; 37C, 5% CO2. After thawing the viability of the cells may be low. |
| Properties | virus replication.; Susceptible to: hepatitis B |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with AST, MD, MPI |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1985;45:1187-1197 - PMID: 2578876
Science 1983;222:385-389 - DOI: 10.1126/science.6194563 - PMID: 6194563 |