Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | MES-SA |
Accession number | ICLC HTL96008 |
Brief description | Species: human, Caucasian; Tissue: uterus; Tumor: sarcoma |
Description | Species: human, Caucasian female 56 years old; Tissue: uterus; Tumor: sarcoma |
Depositor | Obtained from ECACC. |
Reference paper | Cancer Res 1983;43:4943-4950 - PMID: 6883344 |
Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
Culture conditions | McCoy's 5A + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2. |
Properties | chemotherapeutic agents; multiple drug-resistant |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by isoenzymes: confirmed as human with AST, MD, MPI |
Tumorigenicity | tumorigenic in nude mice |
Karyology | modal chromosome number 45 |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |