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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | MCF7-490X1 |
| Accession number | ICLC HTL96002 |
| Brief description | Species: human, Caucasian; Tissue: breast; Tumor: adenocarcinoma; Transfected with: ER; Original line: MCF7 |
| Description | Species: human, Caucasian female 69 years old; Tissue: breast; Tumor: adenocarcinoma; Transfected with the exon 4 defective variant of human estrogen receptor cDNA, cloned in pZeoSV vector, resistant to zeocin; Original line: MCF7; Derived from: pleural effusion |
| Depositor | Dr U. Pfeffer, Molecular Biology Dept, IST at ABC, Genoa, Italy. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2. |
| Properties | variant estrogen receptor transgene |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with G6PD, AST |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |