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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | COLO 320DMF |
| Accession number | ICLC HTL95027 |
| Brief description | Species: human; Tissue: colon; Tumor: adenocarcinoma |
| Description | Species: human female 55 years old; Tissue: colon; Tumor: adenocarcinoma |
| Depositor | Obtained from ECACC |
| Reference paper | Cancer Res 1979;39:4914-4924 - PMID: 498117 |
| Morphology and growth | continuous culture, grown semiadherent, morphology undifferentiated, round cells in suspension and loosely adherent cells in monolayer |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6: trypsin/EDTA is not necessary as cells are partly in suspension and partly only loosely adherent; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2 |
| Properties | production of hormones: epinephrine, serotonin, norepinephrine, adrenocorticotropic, parathyroid hormone |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, MPI |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1993;53:5600-5604 - PMID: 8242610
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