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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | HCT-116 |
| Accession number | ICLC HTL95025 |
| Brief description | Species: human; Tissue: colon; Tumor: carcinoma |
| Description | Species: human male; Tissue: colon; Tumor: carcinoma |
| Depositor | Obtained from ECACC |
| Reference paper | Cancer Res 1981;41:1751-1756 - PMID: 7214343 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | McCoy's 5A + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:10 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | J Natl Cancer Inst 1982;69:767-771 - PMID: 6956756
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