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Cell line nameHeLa
Accession numberICLC HTL95023
Brief descriptionSpecies: human, Black; Tissue: cervix; Tumor: carcinoma, epitheloid
DescriptionSpecies: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid
DepositorObtained from ECACC
Reference paperCancer Res 1952;12:264 - DOI: 10.1016/0006-291x(75)90293-4
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3x104 cells/cm2; 37C, 5% CO2
Propertiesantitumour testing; transformation; tumorigenicity; cytotoxicity; bacterial invasiveness; virology; Susceptible to: adenovirus 3, measles, poliovirus 1, echovirus, vaccinia, arbovirus, respiratory syncytial virus, reovirus 3, rhinovirus, Coxsackie
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with AST, G6PD, MD
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyJ Exp Med 1953;97:695 - DOI: 10.1016/0006-291x(75)90293-4 Obstet Gynecol 1971;38:945-949 - PMID: 4942173

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