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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | HeLa |
| Accession number | ICLC HTL95023 |
| Brief description | Species: human, Black; Tissue: cervix; Tumor: carcinoma, epitheloid |
| Description | Species: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid |
| Depositor | Obtained from ECACC |
| Reference paper | Cancer Res 1952;12:264 - DOI: 10.1016/0006-291x(75)90293-4 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3x104 cells/cm2; 37C, 5% CO2 |
| Properties | antitumour testing; transformation; tumorigenicity; cytotoxicity; bacterial invasiveness; virology; Susceptible to: adenovirus 3, measles, poliovirus 1, echovirus, vaccinia, arbovirus, respiratory syncytial virus, reovirus 3, rhinovirus, Coxsackie |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with AST, G6PD, MD |
| Tumorigenicity | tumorigenic in nude mice |
| Karyology | aneuploid |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | J Exp Med 1953;97:695 - DOI: 10.1016/0006-291x(75)90293-4
Obstet Gynecol 1971;38:945-949 - PMID: 4942173
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