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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | MCF7 |
| Accession number | ICLC HTL95021 |
| Brief description | Species: human, Caucasian; Tissue: breast; Tumor: adenocarcinoma |
| Description | Species: human, Caucasian female 69 years old; Tissue: breast; Tumor: adenocarcinoma; Derived from: pleural effusion |
| Depositor | Dr U. Pfeffer, Molecular Biology Dept, IST at ABC, Genoa Italy. |
| Reference paper | J Natl Cancer Inst 1973;51:1409-1416 - DOI: 10.1093/jnci/51.5.1409 - PMID: 4357757 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2. |
| Viruses | type C, type B |
| Properties | estrogen receptor positive; specialized product secretion; antitumour testing; cytotoxicity; chemosensibility; chemotherapeutic agents; receptor; hormone sensibility; tumorigenicity |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with G6PD, NP |
| Tumorigenicity | tumorigenic in nude mice |
| Karyology | hypertriploid to hypotetraploid |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1990;118:48 - PMID: 2152773
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| Comments | The viability may be low after thawing; thaw with medium supplemented with 20% FBS |