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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | HeLa S3 |
| Accession number | ICLC HTL95020 |
| Brief description | Species: human, Black; Tissue: cervix; Tumor: carcinoma, epitheloid |
| Description | Species: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid; Derived from: HeLa |
| Depositor | Obtained from ECACC. |
| Reference paper | J Exp Med 1956;103:273 - DOI: 10.1016/0006-291x(75)90293-4 |
| Morphology and growth | continuous culture, grown semiadherent, morphology epithelial |
| Culture conditions | Ham's F12 + 10% FBS + 2mM L-Glutamine; Semiadherent cell line, adaptable to growth in suspension (spinner) culture. Maintain culture between 3 and 9x105 cells/ml; split 1:2-1:4. 37C, 5% CO2 |
| Properties | toxicity screening; virology; bacterial adhesiveness; bacterial invasiveness; enzymology; tumorigenicity; Susceptible to: adenovirus 5, poliovirus 1, vesic. stomatitis (Indiana) |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with AST, G6PD, MD, NP, PepB |
| Tumorigenicity | non tumorigenic |
| Karyology | modal number 68 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |