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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | HeLa 432 |
| Accession number | ICLC HTL95016 |
| Brief description | Species: human, Black; Tissue: cervix; Tumor: carcinoma, epitheloid; Transfected with: ER-pBact; Original line: HeLa |
| Description | Species: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid; Transfected with the exon 4 defective variant of ER-pBact cDNA, human estrogen receptor cDNA, under control of the human beta actin promotor, resistant to G418S.; Original line: HeLa |
| Depositor | Dr. U. Pfeffer, Molecular Biology Dept IST at ABC, Genoa, Italy. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:2-1:6 using trypsin/EDTA; seed at 1-3x104 cells/cm2; 37C, 5% CO2. |
| Properties | variant estrogen receptor transgene |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with G6PD, NP |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |