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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SH-SY5Y |
| Accession number | ICLC HTL95013 |
| Brief description | Species: human, Caucasian; Tumor: neuroblastoma |
| Description | Species: human, Caucasian female 4 years old; Tumor: neuroblastoma; Derived from: SK-N-SH, originally derived from metastasis to bone marrow |
| Depositor | Obtained from ECACC. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | MEM (EBSS) + Hams F12 (1:1) + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1x103-1x104 cells/cm2. 37C, 5% CO2. |
| Properties | neurotransmitter studies; convert glutamate to GABA; dopamine-beta-hydroxylase activity |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with AST, NP |
| Passage number | 14 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1973;33:2643-2652 - PMID: 4748425
Cancer Res 1978;38:3751-3757 - PMID: 29704 Cancer Res 1990;50:3422-3428 - PMID: 2334938 |