Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | COR-L23 |
Accession number | ICLC HTL95012 |
Brief description | Species: human, Caucasian; Tissue: lung; Tumor: carcinoma, large cell |
Description | Species: human, Caucasian male 62 years old; Tissue: lung; Tumor: carcinoma, large cell; Derived from: pleural effusion |
Depositor | Obtained from ECACC. |
Reference paper | Br J Cancer 1985;52:495-504 - DOI: 10.1038/bjc.1985.220 - PMID: 2998422 |
Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2 |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
Passage number | 17 |
Tumorigenicity | tumorigenic in nude mice |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
Further bibliography | Br J Cancer 1985;52:21-29 - DOI: 10.1038/bjc.1985.143
- PMID: 3925974
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