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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | A-704 |
| Accession number | ICLC HTL95007 |
| Brief description | Species: human; Tissue: kidney; Tumor: adenocarcinoma |
| Description | Species: human male 78 years old; Tissue: kidney; Tumor: adenocarcinoma |
| Depositor | Obtained from ECACC. |
| Reference paper | J Natl Cancer Inst 1973;51:1417-1423 - DOI: 10.1093/jnci/51.5.1417 - PMID: 4357758 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-2x105 cells/cm2. 37C, 5% CO2. |
| Properties | chemotherapeutic agents; cytotoxicity; tumorigenicity |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, MPI |
| Passage number | 53 |
| Tumorigenicity | non tumorigenic |
| Karyology | diploid to hyperdiploid |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Oncogene 1994;9(3):939-942 - PMID: 8108138
Proc Natl Acad Sci USA 1987;84(22):7972-7976 - DOI: 10.1073/pnas.84.22.7972 - PMID: 2825172 |