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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | U-937 |
| Accession number | ICLC HTL94002 |
| Brief description | Species: human, Caucasian; Tissue: pleural effusion; Tumor: lymphoma, histiocytic |
| Description | Species: human, Caucasian male 37 years old; Tissue: pleural effusion; Tumor: lymphoma, histiocytic |
| Depositor | Obtained from ATCC. Established by C. Sundrom and K. Nilsson in 1974. |
| Reference paper | Int J Cancer 1976;17:565-577 - DOI: 10.1002/ijc.2910170504 - PMID: 178611 |
| Morphology and growth | continuous culture, grown in suspension, morphology histomonocytoid |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split 1:5-1:20, maintain cultures between 2 and 9x105 cells/ml. 37C, 5% CO2. |
| Properties | IL-1 production; specialized product secretion; tumor markers; Fc receptors; antitumour testing; virology; tumorigenicity; expression of markers and properties of monocytes, expression of mRNA for c-myc.; Susceptible to: HIV-1, HIV-2 |
| Distribution | Cell line available for distribution. The cell line must not be exploited commercially |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with AST, NP, PepB |
| Validated by HLA-DP beta: confirmed as human by PCR | |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Amelogenin: X; vWA: 14,15,16,17; FGA: 22,25; TH01: 6,9.3; D18S51: 13,14; D21S11: 27,29; D8S1179: 12,13 |
| Further bibliography | J Exp Med 1976;143:1528-1533 - DOI: 10.1084/jem.143.6.1528
- PMID: 1083890
J Immunol 1980;125:463-465 - PMID: 7381208 Nature 1979;279:328-331 - DOI: 10.1038/279328a0 - PMID: 450085 |