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IRCCS Ospedale Policlinico San Martino
Interlab Cell Line Collection (ICLC)
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Cell line nameK-562
Accession numberICLC HTL94001
Brief descriptionSpecies: human, Caucasian; Tissue: pleural effusion; Tumor: leukemia, myelogenous
DescriptionSpecies: human, Caucasian female 53 years old; Tissue: pleural effusion; Tumor: leukemia, myelogenous
Depositor Dr V. Dessi, San Martino Hospital, Genoa, Italy
Reference paperBlood 1975;45:321-334 - PMID: 163658
Morphology and growthcontinuous culture, grown in suspension, morphology round and large cells
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Maintain cultures between 1 and 5x105 cells/ml; 37C, 5% CO2
Propertieshaemoglobin synthesis; antitumour testing; tumorigenicity; differentiation; pharmacodynamics; cloning; cytotoxicity; cellular effects of hyperthermia; natural killer assay
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with AST, NP, Pep B
Validated by HLA-DP beta: confirmed as human by PCR
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyBlood 1980;56:344-350 - PMID: 6996765
Clin Haematol 1984;13:461-486 - PMID: 6088146
J Immunol 1982;129:2504-2510 - PMID: 6982930

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